On 26 April 2021, Lost Marble LLC released Moho 13.5, with new features including Vitruvian Bones, Wind Dynamics and Quad meshes, as well as a slightly refreshed User Interface. On 22 December 2020, it was announced that the Moho 2D animation software had been acquired by Lost Marble LLC, a company founded by Mike Clifton, the original creator of Moho, and Victor Paredes, Supervisor of Moho animation at Cartoon Saloon and former Moho Product Manager. In June 2019, Moho 13 introduced new bitmap tools, including bitmap frame to frame capabilities, and re-engineered 3D objects support. This feature set enabled users to create animations more quickly and easily through functions such as a new overlay timeline, rigged characters, rotating canvases and frame-by-frame animation. Smith Micro worked with Microsoft to develop Moho functionality for the Surface Dial peripheral. In October 2016, Moho became available for the Microsoft Surface Studio. In 2016, Moho 12 was released with Pin Bones, optimized Bézier handles, improved free hand tools, Smart Warp, real motion blur, and more. In 2016, Anime Studio was renamed Moho by Smith Micro Software to reflect the software's ability to do more than just anime. In 2015, Anime Studio 11 added frame-by-frame animation, layer referencing, animated shape ordering, enhanced tools and brushes, JSON file format support, and other features. In 2014, Anime Studio 10 contained upgraded features and new mechanics to its predecessor. In 2012, Smith Micro released Anime Studio 9, with new features including Smart Bones, Editable Motion Graphs, and Bézier handles, as well as enhancements to the timeline, key frames, and onionskins. Version 8.1 also supported the new Poser 9 SDK and integrated the Wacom multi-touch API, allowing it to work natively with Wacom's Bamboo and Intuos tablets. In 2011, Anime Studio 8 added features such as the Character Wizard, layered Photoshop import, and real-time media connection. In 2010, Smith Micro released Anime Studio 7, which added features such as Physics, 3D creation and an improved interface.

The software was distributed by E Frontier until 2007, when it was acquired by Smith Micro and renamed Anime Studio, as a marketing companion for the former Manga Studio. The software was originally developed under the name 'Moho' in 1999 by Mike Clifton at Lost Marble. It has two different packages: Moho Pro and Moho Debut, similar to the pro version but with features restricted. Moho (previously marketed as Anime Studio) is a proprietary vector-based 2D animation application distributed by Lost Marble LLC.

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